Evaluation the Genotoxicity of PHB Nanoparticle by Micronucleus Assay

Abstract

Determining the toxicity of substances in vivo is one of the most important tests that judge whether or
not they are used in the pharmaceutical field. In the present study, the genetic toxicity of treatment doses
of Polyhydroxybutyrate (PHB), PHB nanoparticles, Cefotaxime and complex of PHB nanoparticlesand
cefotaxime was evaluated. The effect of these substances on the number and percentage of white blood
cells (WBCs) in mice was also tested (in vivo). Micronucleus assay was used to assess genotoxicity of
above materials in vivo, as well as the technique of WBCs chamber was used to estimate the total number
of WBCs in mice administrated with above substances. The differential count of WBCs was measured
by staining the smears with leishman stain. The present study demonstrated that there were no significant
differences (P>0.05) in the number of micronucleus cells in the mice injected with treatment doses of PHB
nanoparticles, Cefotaxime and complex of PHB nanoparticlesand cefotaximewhen compared with mice
injected with normal saline. Similar finding was obtained in terms of counting of total WBCs and differential
count in mice injected with treatment doses of PHB, PHB nanoparticles, Cefotaxime and complex of PHB
nanoparticlesand cefotaxime when compared with WBCs total count and differential count in mice injected
with normal saline (P>0.05). It can be concluded that there is no toxic effect of treatment doses of PHB, PHB
nanoparticles, Cefotaxime and complex of PHB nanoparticlesand cefotaxime on mice.