Effect Phenytoin Therapy to Fibroblasts and Angiogenesis of Enterocutaneous Fistula in Wistar Rat

Authors

  • Muhammad Budiman Irpan Bachtiar
  • Hermawan
  • Hardian
  • Ignatius Riwanto

DOI:

https://doi.org/10.37506/ijfmt.v15i4.16901

Keywords:

Enterocutaneous fistulas, phenytoin, fibroblasts, angiogenesis

Abstract

Background: Comprehensive wound care in enterocutaneous fistula (ECF) is the therapeutic of choice
currently, which may increase of closure rate without surgery from 19 to 92%. Phenytoin has been reported
have anticolagenase effect on wound healing is hoped to improve the ECF closure.
Aim: The study was aimed to demonstrate the effect of phenytoin on closure of enterocutaneous fistula seen
from the number of fibroblasts and angiogenesis
Methods: This study was “Randomized Controlled trial with post test only group design” on 18 male
wistar rats with ECF, that were divided randomly into three groups: group(K) control, P1(topical phenytoin),
P2(oral phenytoin). After 7 days of treatment, they were terminated and histopathological examinations were
performed to do fibroblast cell counting and the amount of angiogenesis by Hematoxilin Eosin staining.
Results: Mean of the number of fibroblastin groups K, P1 and P2 were 69.50 ± 10.07, 155.50 ± 13.50 and
182.16 ± 11.85 respectively (One way Anova P=0.001) and mean of the number of angiogenesis in groups
K, P1, and P2 were 95.66 ± 9.72, 178.66 ± 11.75, and 205,16 ± 9.74 respectively (One way Anova P=0.001).
Post Hoc Test LSD showed thatthe number of fibroblast of group P1 vs K(P=0.001), P2 vs K(P=0.001),
P1 vs P2(P=0.004) and the number of angiogenesis of group P1 vs K(P=0.001), P2 vs K(P=0.001), P2 vs
P1(P=0.002).
Conclusion: The therapy of topical and oral phenytoin increased the number of fibroblasts and angiogenesis
in Wistar rat with ECF. Out come of oral phenytoin therapy better than topical.

Author Biographies

Muhammad Budiman Irpan Bachtiar

Master Biomedical Student at Diponegoro University Biomedical Master’s Student

Hermawan

Lecture at Departement of Anatomic Pathology, Faculty of Medicine, Diponegoro University,

Hardian

Lecture at Department of Physiology,Faculty of Medicine, Diponegoro University,

Ignatius Riwanto

Lecture at Department of Digestive Surgery, Faculty of Medicine,Diponegoro University

Published

2021-08-16

How to Cite

Muhammad Budiman Irpan Bachtiar, Hermawan, Hardian, & Ignatius Riwanto. (2021). Effect Phenytoin Therapy to Fibroblasts and Angiogenesis of Enterocutaneous Fistula in Wistar Rat. Indian Journal of Forensic Medicine & Toxicology, 15(4), 1379-1388. https://doi.org/10.37506/ijfmt.v15i4.16901