Effect Phenytoin Therapy to Fibroblasts and Angiogenesis of Enterocutaneous Fistula in Wistar Rat
DOI:
https://doi.org/10.37506/ijfmt.v15i4.16901Keywords:
Enterocutaneous fistulas, phenytoin, fibroblasts, angiogenesisAbstract
Background: Comprehensive wound care in enterocutaneous fistula (ECF) is the therapeutic of choice
currently, which may increase of closure rate without surgery from 19 to 92%. Phenytoin has been reported
have anticolagenase effect on wound healing is hoped to improve the ECF closure.
Aim: The study was aimed to demonstrate the effect of phenytoin on closure of enterocutaneous fistula seen
from the number of fibroblasts and angiogenesis
Methods: This study was “Randomized Controlled trial with post test only group design” on 18 male
wistar rats with ECF, that were divided randomly into three groups: group(K) control, P1(topical phenytoin),
P2(oral phenytoin). After 7 days of treatment, they were terminated and histopathological examinations were
performed to do fibroblast cell counting and the amount of angiogenesis by Hematoxilin Eosin staining.
Results: Mean of the number of fibroblastin groups K, P1 and P2 were 69.50 ± 10.07, 155.50 ± 13.50 and
182.16 ± 11.85 respectively (One way Anova P=0.001) and mean of the number of angiogenesis in groups
K, P1, and P2 were 95.66 ± 9.72, 178.66 ± 11.75, and 205,16 ± 9.74 respectively (One way Anova P=0.001).
Post Hoc Test LSD showed thatthe number of fibroblast of group P1 vs K(P=0.001), P2 vs K(P=0.001),
P1 vs P2(P=0.004) and the number of angiogenesis of group P1 vs K(P=0.001), P2 vs K(P=0.001), P2 vs
P1(P=0.002).
Conclusion: The therapy of topical and oral phenytoin increased the number of fibroblasts and angiogenesis
in Wistar rat with ECF. Out come of oral phenytoin therapy better than topical.
Downloads
Published
How to Cite
Issue
Section
License
- The journal allows readers to read, download, copy, distribute, print, search, or link to the full texts of its articles and allow readers to use them for any other lawful purpose.
- The journal allows the author(s) to hold the copyright without restrictions.
- The journal allows the author(s) to retain publishing rights without restrictions