Molecular Characteristics of Iraqi Lactobacillus plantarum Isolates and Evaluate its Natural Bacteriocin(plantaricins) Antimicrbial Activity against Pathogenic Bacteria

Authors

  • Khadeeja S. Madhi
  • Mohammed H. Khudr
  • Rasha M. Othman

DOI:

https://doi.org/10.37506/ijfmt.v15i4.16931

Keywords:

Lactobacillus plantarum, molecular,bacteriocin, antimicrobial, pathogenic bacteria

Abstract

In this study, local strains of Lactobacillus plantarum bacteria were isolated from traditional Iraqi raw
milk and milk products. The present study was aimed to study the molecular aspects of Iraqi Lactobacillus
plantarum isolates and to evaluate its natural plantaricins antimicrobial activity against pathogenic bacteria
. For complete detection for Lactobacillus spp. the partial sequencing of 16S rDNA gene result was analyzed
and compared with those in GenBank to find out the different in the sequence using the BLAST program
(http:// www. ncbi. nlm.nih). The result was 17 samples diagnosed as Lactobacillus plantarum. Moreover
14 isolates of local Lactobacillus plantarum bacteria were show 100% similarity with those previously
recorded in GenBank, while 3 isolates were found to display more than 99% similarity with L. plantarum
strains were previously listed in GenBank. Moreover the study also reveals that all L.plantarum strains was
showed antibacterial activity against three studied indicators bacteria and the plnEF gene (530bp) was
detected in all 17 isolates of L.plantarum.

Author Biographies

Khadeeja S. Madhi

College of Veterinary Medicine, University of Basrah, Basrah, Iraq

Mohammed H. Khudr

College of Veterinary Medicine, University of Basrah, Basrah, Iraq

Rasha M. Othman

College of Veterinary Medicine, University of Basrah, Basrah, Iraq

Published

2021-08-16

How to Cite

Khadeeja S. Madhi, Mohammed H. Khudr, & Rasha M. Othman. (2021). Molecular Characteristics of Iraqi Lactobacillus plantarum Isolates and Evaluate its Natural Bacteriocin(plantaricins) Antimicrbial Activity against Pathogenic Bacteria. Indian Journal of Forensic Medicine & Toxicology, 15(4), 1563-1573. https://doi.org/10.37506/ijfmt.v15i4.16931