Effect of Exotoxin a isolated from Pseudomonas Aeruginosaagainsthuman Cancer Cell Lines

Authors

  • Muthanna Hamid Hassan

DOI:

https://doi.org/10.37506/ijfmt.v15i4.16972

Keywords:

p.aeruginosa, exotoxin A, chromatography ,cancer cell line

Abstract

In this study, the presence of Pseudomonasaeruginosawas investigated from many clinical samples, and
then about 54 isolates were obtained, diagnosed with all different diagnostic methods, and then the bacterial
isolates produced for ExotoxinA were detected by the ELISA method and then the most productive isolate
was chosen for purification by means ofPrecipitation with ammonium sulphate and ion-exchange column
and detection of molecular weight was (M.W 65.03KD).The competent isolation tested its toxicity against
two cancer cell linesHeLa and PC3 which were an inhibition ratio 69.1% and 61.6% respectively in high
concentration.

Author Biography

Muthanna Hamid Hassan

Assist. Prof., Department of biology, College of Science, university of Anbar, Iraq

Published

2021-08-16

How to Cite

Muthanna Hamid Hassan. (2021). Effect of Exotoxin a isolated from Pseudomonas Aeruginosaagainsthuman Cancer Cell Lines. Indian Journal of Forensic Medicine & Toxicology, 15(4), 1853-1859. https://doi.org/10.37506/ijfmt.v15i4.16972