Effect of Exotoxin a isolated from Pseudomonas Aeruginosaagainsthuman Cancer Cell Lines
Keywords:p.aeruginosa, exotoxin A, chromatography ,cancer cell line
In this study, the presence of Pseudomonasaeruginosawas investigated from many clinical samples, and
then about 54 isolates were obtained, diagnosed with all different diagnostic methods, and then the bacterial
isolates produced for ExotoxinA were detected by the ELISA method and then the most productive isolate
was chosen for purification by means ofPrecipitation with ammonium sulphate and ion-exchange column
and detection of molecular weight was (M.W 65.03KD).The competent isolation tested its toxicity against
two cancer cell linesHeLa and PC3 which were an inhibition ratio 69.1% and 61.6% respectively in high
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