Detection of Enterotoxigenic Staphylococcus aureus in Patients with Gastroenteritis in Erbil/Iraq
Keywords:Gastroenteritis, Staphylococcus aureus, sea and seb Enterotoxin genes.
Gastroenteritis is one of the words serious public health problem, and enterotoxigenic Staphylococcus aureus
is one of the causes of gastroenteritis. The aim of this study was the molecular detection of enterotoxigenic
genes in Staphylococcus aureus, then detection the association of resistance patterns with the toxigenicity
of strains. Microbiological analysis of fecal samples from patients with gastroenteritis was performed for
detection of S. aureus. The samples were cultured and identified by routine bacteriological methods and
VITEK 2 system. Subsequently PCR amplification of enterotoxin genes (sea and seb) was carried out
on all S. aureus strains. The results indicated that in total of 417 fecal samples taken from patients with
acute gastroenteritis, 30 grew S. aureus (7.19%), and of 417 fecal samples 96 yielded bacterial pathogens
(23.02%), S. aureus was the most common bacterial pathogen found in 30/96 cases (31.25%). In 30 isolated
of S. aureus, penicillin sensitive S. aureus were 2 (6.67%), methicillin sensitive S. aureus were 19 (63.33%),
multidrug resistant S. aureus were 12 (40%), methicillin resistant S. aureus were 11 (36.67%), Extensive
drug resistance were 3 (10%), and none of them was pan drug resistant,and all isolates were sensitive to
vancomycin. The PCR results showed that 7/30 (23.33%) of the S. aureus isolates possessed the sea gene,
and 3/30 (10 %) had the seb gene. In total of 30 isolates Staphylococcal enterotoxin genes sea and seb were
more prevalent among methicillin resistant S. aureus were 6/11 (54.54%), 3/11 (27.27%) respectively, then
in multidrug resistant S. aureus which were 6/12 (50%), and 3/12 (25%) respectively, and in Extensively
drug resistance S. aureus sea was1/3 (33.33%) and there is no seb genes, while in methicillin sensitive S.
aureus sea was 1/19 (5.26%), and there is no seb genes, and there was no Staphylococcal enterotoxin genes
sea and seb in penicillin sensitive S. aureus. The result confirmed the involvement of enterotoxigenic S.
aureus in the occurrence of gastroenteritis, and isolates with enterotoxin genes have a higher drug resistance
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