Genotyping of Histological Cutaneous Leishmaniasis Isolated by Dental Broach Smear in Iraqi Patients
Keywords:Cutaneous leishmaniasis, genotypes, Al-Karama Hospital, PCR, histopathology.
Cutaneous leishmaniasis is a major public health problem as a disease endemic in Iraqi population. Many
diagnostic tools are used to establish the diagnosis like smear, histopathology and culture. To find more
rapid, sensitive and specific diagnostic method for the diagnosis of cutaneous leishmaniasis, and to detect
the genotypes of leishmania tropica and leishmania major strains in Iraq. Sixty six patients (43 males and
23 females) with clinical diagnosis of cutaneous leishmaniasis were included in present work during the
period between December / 2019 to May / 2020 in Al-Yarmook Teaching Hospital and Al-Karama Teaching
Hospital. The age of patients ranged from 6 months to 55 years, with median age 24 years. The following
diagnostic techniques were carried out for diagnosis of cutaneous leishmaniasis including dental broach
smear, histopathology examination and culture on Roswell park medium institute (RPM I 1640) and on
NNN media. In addition to polymerase chain reaction (PCR) and restriction fragment length polymorphism
(RFLP), genotype techniques were performed for all patients. Sixty patients with other skin lesions were
processed for PCR as a control group.
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