Determination the Relationship between (KLF14 rs972283) Genotype and Type 2 Diabetes in a Sample of Iraqi Patients

Abstract

This study was aimed to detect KLF14 gene polymorphism in Iraqi type 2 diabetes mellitus patients (T2DM)
to found the correlation between the SNP (rs972283) polymorphism in KLF14 gene and lipid metabolism
and impact on the incidence of type 2 diabetes mellitus (T2DM). The result show high Significant difference
was observed in FBS level (P<0.001) in patient group (172.9 ± 79.73) and in control (81.16 ± 7.18),).
Cholesterol, LDL-Cholesterol and VLDL-Cholesterol mean level value in diabetic patients was significantly
higher than those of control group (p<0.0001), serum HDL-Cholesterol mean value was significantly
difference (P<0.05) in serum mean level of HDL between T2DM patients and healthy controls. Real time
PCR (HRM) RT-PCR were used to detect SNP (rs972283) in KLR14 gene (G>A) by using specific primers,
as a related with SNP (rs972283) G>A in KLF14 gene, genotypes and alleles frequencies, odds ratios,
95% confidence intervals and P values for the KLF14 gene In this study, statistical analyses of genotypic
frequencies for the KLF14 (rs972283) revealed significant difference between T2DM patients and controls
in the examined population. GG genotype was significantly (P = 0.0006) more frequent in the patient group.
The observed G/G, A/G, and A/A genotype frequencies were 50%, 42%, and 8%, respectively
.The A (wildtype)
and G (variant) allele frequencies were 29% and 71% , respectively in patient group while the observed
G/G, A/G, and A/A genotype frequencies were 22%, 48%, and 30, respectively
and the A (wild-type) , G
(variant) allele frequencies were 54% and 46%, respectively in control group (P <0.0003). GG genotype was
significantly (P = 0.0006) more frequent in the patient group, Conclusion: There was a relationship between
polymorphism of KLF14 gene SNP (rs972283) and the incidence of T2DM in sample of Iraqi patients.