The SNP rs13118928, rs1828591 and rs10519717 in the HHIP Gene are not Associated on COPD Susceptibility in Male Javanese Smokers

Abstract

Background: Hedgehog Interacting Protein (HHIP) gene polymorphisms have an association on COPD
has been carried out in Europe and Asia but in Indonesia there is still very limited study on this type and
the largest ethnic group in Indonesia is the Javanese. Objective: To analyze the association between the
HHIP gene polymorphism and the incidence of COPD in male Javanese smokers in Lampung, Indonesia.
Method: In a case-control study in Javanese male smokers, three single nucleotide polymorphism (SNPs)
in the HHIP gene were analyzed by Sanger sequencing method. There were 110 participants in this study
which were divided into 2 groups, such as COPD group (55 participants) and control group (55 participants).
Three SNPs in the gene (rs13118928, rs1828591 and rs10519717) were selected for genotyping. Genotype
distributions were compared between patients and controls. The statistical analysis was carried out with the
SPSS program with a chi-square test. Result: The genotypic frequency of the HHIP gene sequence at the
SNP position rs1828591, such as AA (52.72%), GG (3.63%) and AG (43.63%) in COPD group, while in
the control group such as AA (38.18%), GG (9.09%) and GG (52.72%; p > 0.05). The genotypic frequency
of the HHIP gene sequence at the SNP position rs13118928 consisted of AA (47.27%) and AG (53.72%)
in the control group, while the COPD group consisted of AA (52.72%), GG (1.81%) and AG (45.45%; p
> 0.05). The genotypic frequency of the HHIP gene sequence at the SNP position rs10519717 consisted
of TT (34.54%), CC (14.56%) and CT (50.90%) in COPD group, while controls group consisted of TT
(23.63%), CC (16.37%) and CT (60.00%; p > 0.05). The genotypic analysis of Three SNPs in HHIP gene
were observed but showed no significant difference between case and control groups. Conclusion: Singlenucleotide
variants in the HHIP gene are not associated with COPD susceptibility in Javanese male smokers.