Decreased Antioxidant Capacity in Corn Farmers Occupationally Exposed to the Mixture of Herbicides

Authors

  • Unchisa Intayoung
  • Kanyapak Kohsuwan
  • Klintean Wunnapuk
  • Ratana Sapbamrer
  • Supakit Khacha-Ananda

DOI:

https://doi.org/10.37506/ijfmt.v15i4.17121

Keywords:

8-isoprotane, Glyphosate, Paraquat, Total antioxidant capacity (TAC)

Abstract

Background: The application of herbicides has been a common practice in worldwide agriculture, mainly
with the goal to increase efficacy of weed control. So, most farmers faced a greater risk of herbicide
exposure to develop adverse health effects from oxidative stress-induced herbicides. The aim of this study
is to determine the urinary total antioxidant capacity (TAC) and 8-isoprostane levels in farmers using the
mixture of herbicides in Long District, Phrae Province, Thailand.
Methods: Ninety-three participants were recruited. The spot urine samples (pre- and post-work) were
collected. The urinary TAC was determined using ABTS radical scavenging assay. The urinary 8-isoprostane
level was investigated by commercial ELISA kit.
Results: Most farmers worked on a farm during 1-5 h/day. Fifty-five percents of participants used the
mixture of herbicide while working between glyphosate and paraquat as well as glyphosate and 2,4-D. The
urinary TAC levels in pre-work urine sample of farmers applied combining herbicides were significantly
higher than that level in their post-work urine sample. However, there was no significant difference between
pre- and post-work urinary 8-isoprostane in farmers applied combining herbicides.
Conclusion: The results suggest that occupational exposure to mixture of glyphosate herbicides plus paraquat
or 2,4-D could induce abnormal oxidative stress value especially antioxidant defense among agricultural
workers.

Author Biographies

Unchisa Intayoung

Graduate Student, Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand, 50200

Kanyapak Kohsuwan

Graduate Student, Chiang Mai University, Chiang Mai, Thailand, 50200

Klintean Wunnapuk

Assistant Professor, Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand, 50200

Ratana Sapbamrer

Associate Professor, Department of Community Medicine, Chiang Mai University, Chiang Mai, Thailand, 50200

Supakit Khacha-Ananda

Instructor, Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand, 50200

Published

2021-08-16

How to Cite

Unchisa Intayoung, Kanyapak Kohsuwan, Klintean Wunnapuk, Ratana Sapbamrer, & Supakit Khacha-Ananda. (2021). Decreased Antioxidant Capacity in Corn Farmers Occupationally Exposed to the Mixture of Herbicides. Indian Journal of Forensic Medicine & Toxicology, 15(4), 2763-2770. https://doi.org/10.37506/ijfmt.v15i4.17121