Prevalence of Cryptosporidium spp. among Patients with Diarrhea at Wasit Province/ Iraq
Keywords:Cryptosporidium, M.Z.N stain, multiplex PCR Cryptosporidiosis.
Cryptosporidium is an important obligate enteric protozon parasite that infects humans and wild range
of animals with morbidity and mortality especially amongimmuno-suppressed individualsThis study
carried out in al-kut city /Iraq in two major hospital Al- kut hospital for gynecology ,obstetric and
pediatric and al-Karamma teaching hospital which included collection of stool samples from diarrheal
patients from October / 2020 – January / 2021 , (109) stool samples taken from patients with different
ages to both gender examined by microscopic examination(Modified ziehl nelson) and multiplex PCR
technique, the results showed the percentage of cryptosporidium spp. infection by using M.Z.N stain
was 44(40.4%) while by using PCR technique was 38(39.6%) and 4(4.2%) for C.parvum and C.hominis
The rate of infection is significantly associated with residence when using M.Z.N stain and PCR for
C.parvum, also significant associate among those who >5 years old. water source for drinking were
significant associated factors, other factors (gender, education level) Showed no significant association
with infection, the present study aimed to detection of Cryptosporidium spp. and possible association
between the infection and some risk factors by using microscopic examination and detecting DNA of
Cryptosporidium parasite by multiplex PCR technique.
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