The Cytotoxic Effect of Iraqi Rumex Acetosella against Breast and Esophagus Cancer Cells

Authors

  • Omar Hussein Ahmed
  • Ali Jabbar Abdulhussein
  • Enas Jawad Kadhim

DOI:

https://doi.org/10.37506/ijfmt.v16i1.17616

Keywords:

Medicinal Plants, Phytochemicals, Natural Compounds, anticancer Activity.

Abstract

Background: cell lines derived from cancer cells are frequently used in research, including use as a model to understand cancer and to identify potential new treatments. The aim of this article has been prepared to evaluate the anticancer effect of Rumex Acetosella that has been studied for their anticancer activity on esophagus and breast cancer cell line. Method: AMJ13 (new breast cancer cell line (AMJ13) has been established from an Iraqi breast cancer patient and SK-GT-4 cells (Human esophageal adenocarcinoma cell line) were treated with whole methanolic extract of the leaves of Rumex acetosella. the MTT assay to determine the anticancer activity was done using 96-well plates where cell lines were seeded at 1 × 104cells/well after 24 hrs.m the cells were treated with tested compounds at different concentrations. Cell viability was measured after 72 hrs of treatment by removing the medium, adding 28 μL of 2 mg/mL solution of MTT, and incubating the cells for 2.5 h at 37 °C. After removing the MTT solution, the crystals remaining in the wells were solubilized by the addition of 130 μL of DMSO (Dimethyl Sulphoxide) followed by 37 °C incubation for 15 min with shaking. The absorbency was determined on a microplate reader at 492 nm. Conclusion: Plant extract from Rumex Acetosella showed particularly strong anticancer capabilities since it inhibited actual tumor progression in a breast adenocarcinoma mouse model. Our results suggest that whole plant extracts are promising anticancer reagents.

Author Biographies

Omar Hussein Ahmed

Tutor, Pharmacognosy and Medicinal Plants Department, Pharmacy College, Tikrit University, Tikrit, Iraq,

Ali Jabbar Abdulhussein

 Tutor, Pharmacology and Toxicology Department, Pharmacy College, Baghdad, Baghdad University, Iraq,

Enas Jawad Kadhim

Professor, Pharmacognosy and Medicinal Plants Department, Pharmacy College, Baghdad, Baghdad University, Iraq

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Published

2021-11-30

How to Cite

Omar Hussein Ahmed, Ali Jabbar Abdulhussein, & Enas Jawad Kadhim. (2021). The Cytotoxic Effect of Iraqi Rumex Acetosella against Breast and Esophagus Cancer Cells. Indian Journal of Forensic Medicine & Toxicology, 16(1), 929–940. https://doi.org/10.37506/ijfmt.v16i1.17616