Production, Purification and Characterization of Bacteriocin Produced by Novel L. PentosusMW857478 for Enhancement of Food Safety and Shelf-Life of Paneer

Authors

  • Verinder Virk
  • Garima Verma
  • Chand Ram

DOI:

https://doi.org/10.37506/ijfmt.v16i1.17662

Keywords:

Antagonistic activity, Bacteriocin, purification, raw milk, pathogens, lactic acid bacteria

Abstract

This research paper is based upon the production, purification and characterization of bacteriocin by
L.pentosusMW857478 followed by its enhancement of food safety and shelf life of paneer. In recent
year LAB (Lactic acid bacteria) produced bacteriocinattract a great attention of researcher due to
their many potential applications. This paper focused on isolation, identification, evaluation of broad
spectrum inhibitory activity, production and purification, characterization and evaluation of food safety
and shelf life of paneer. Bcateriocin produced byL. pentosusshowedantagonistic activity against food
spoiling pathogens in broad range ofBacteriocin production parameter was optimized with pH 5.5
incubated at 35°C forL. pentosus. Bacteriocin was purified by ammonium sulphate precipitation and
purified bacteriocin with single band on SDS-PAGE for molecular weight. The purified bacteriocin
stableat 2-10 pH and 30-75°C temperatures, suggesting L. pentosusa potent candidates for safety and
extending shelf life of paneer for 15 days.

Author Biographies

Verinder Virk

Assistant Professor, Dept. of Microbiology, KanyaGurukul Campus, GurukulKangri University, Haridwar,India

Garima Verma

Assistant Professor, Dept. of Microbiology, KanyaGurukul Campus, GurukulKangri University, Haridwar,India

Chand Ram

Principal Scientist National Dairy Research Institute, Karnal Haryana India

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Published

2021-11-30

How to Cite

Verinder Virk, Garima Verma, & Chand Ram. (2021). Production, Purification and Characterization of Bacteriocin Produced by Novel L. PentosusMW857478 for Enhancement of Food Safety and Shelf-Life of Paneer. Indian Journal of Forensic Medicine & Toxicology, 16(1), 1219–1228. https://doi.org/10.37506/ijfmt.v16i1.17662