Study the Ability of Pseudomonas Aeruginosa Isolated from Different Clinical Cases to Biofilm Formation and Detection of Algd Gene

Authors

  • Hussamsalah al-deen
  • Suaad Khalil Ibrahim

DOI:

https://doi.org/10.37506/ijfmt.v16i1.17683

Keywords:

Pseudomonas aeruginosa , biofilm , alg D

Abstract

98 samples were collected from various clinical sources included ( Burns, wounds, urines, sputums, blood ) From the city of Baghdad , After performing the biochemical and microscopic examination, 52 isolates were obtained for Pseudomonas aeruginosa , 17 (32.7 %) isolates from burn infection , 12
( 23% ) isolates from Wound infection 11 ( 21.2 % ) isolates from urine infection , 7 ( 13.5 % ) isolates of sputum and 5 ( 9.6 %) isolates from blood . Bacteria susceptibility to form biofilm has been detectedby microtiter plate method, The results showed that 80 % of the bacterial isolates were produced the biofilm with different proportions , alg D gene ( alginate production ) has been detected by polymerase chain reaction ( PCR ) Which plays an essential role in the formation of the biofilm , The PCR results showed that the percentage of gene presence was( 95.4 % )

Author Biographies

Hussamsalah al-deen

Post graduate, Department of Biology, College of Education For pure Sciences / Ibn Al – Haitham University of Baghdad, Baghdad – Iraq

Suaad Khalil Ibrahim

Assistant Pro., Department of Biology, College of Education For pure Sciences / Ibn Al – Haitham University of Baghdad, Baghdad – Iraq

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Published

2021-11-30

How to Cite

Hussamsalah al-deen, & Suaad Khalil Ibrahim. (2021). Study the Ability of Pseudomonas Aeruginosa Isolated from Different Clinical Cases to Biofilm Formation and Detection of Algd Gene. Indian Journal of Forensic Medicine & Toxicology, 16(1), 1368–1374. https://doi.org/10.37506/ijfmt.v16i1.17683