Tobacco Smoking as A Risk Factor in DNA Methylation of Repair Gene (MLH1) Using Cytobbrush from Lateral Border of the Tongue
Keywords:This study concluded that smoking considered as a risk factor predisposed to process of oral carcinogenesis.
Objective: The aim of this study was to evaluate the epigenetic effect in the process of oral carcinogenesis
by screening the methylation of repair gene in chronic tobacco smokers.
Material and Method: Study design: One hundred male volunteers, divided into two groups: the 1st
group consisted of 58 smokers, each consumed 20 cigarettes/day for at least 10 years; and the 2nd group
consisted of 48non-smokers who were consider as a control group.
The samples were taking from lateral border of the tongue by exfoliative cytology, and the extracted
DNA was treated with phenol-chloroform gDNA,Screening of methylation was done by Methyl
Specific PCR (MSP).
Results :- The results of this studyshowed significant effect of tobacco smoking in methylation of
MLH1 gene in site 1in comparison to non-smoker group,(P > 0.05), with Odds ratio = 4.957 CI ().
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