Molecular detection of Brucella canis in Blood of Dogs
Keywords:Brucella canis, dogs, Iraq
The aims of this study was to evaluate a PCR for detecting Brucella canis in the blood of dogs, using a
primer pair designed for Brucella spp.
A study was conducted on 150 blood sample collected from dogs suspected to Veterinary Hospital
in Baghdad / Aden Square. All blood samples (150) were tested by PCR technique using a common
primer of the 23S ribosomal RNA (23s RNA) gene and specific primer for brucella canis (B0548).
The genomic DNA was extracted and PCR was applied. Our study recorded 5.3% of brucellosis in
common primer and 3.3% in specific primer for brucella canis in dog in Baghdad city, the sequences
of Brucella canis in dog in different isolates in our study recorded 99% compatibility recording to
National Center Biotechnology Information (NCBI). Following correspondence from National Center
for Biotechnology Information, the 23S ribosomal RNA gene was registered, given an agreement
number, and became a resource for Iraq and Middle East, as well as the rest of the world. As more type
strains are published, this set will grow, and it can be download from NCBI at: https://www.ncbi.nlm.
From this study we can conclude that, the percentage of Brucellosis in dogs in Baghdad city is 5.3% and
3.3% in a common and specific primer, respectively and the molecular method (PCR), is a good idea
for confirmation of diagnosis of Brucella canis infection in dog.
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