Thiopurine S-Methyltransferase Genotyping in Iraqi Childhood Acute Lymphoblastic Leukemia Patients ; A Single Institute Study

Authors

  • Esraa Ali Kadhum
  • Manal K Rasheed
  • Hasanein H.Ghali

DOI:

https://doi.org/10.37506/ijfmt.v16i1.17805

Keywords:

6-Mercaptopurine , Acute lymphoblastic leukemia, neutropenia ,drug -toxicity

Abstract

Background: Acute lymphoblastic leukemia (ALL) is the most common cancer seen in children
worldwide and is the most common cancer in children under 14 years of age. Although there have
been major advances in treatment approaches for childhood ALL, serious toxicities such as profound
leukopenia frequently affect treatment and lead to life threatening consequences such as severe
infections and even death. There has been a lot of interest in inter-individual differences in drug
metabolizing enzymes in order to better adjust drug dosage and therapy. In this regards, Thiopurine
S-Methyltransferase (TMPT) was the first pharmacogene that showed a substantial association
with Mercaptopurine (6-MP) maximum tolerated dose and 6-MP related toxicities leading to the
implementation of TPMT genotyping before drug administration Aim: To identify the most common
TPMT polymorphism (TPMT*3A, TPMT*3B and TPMT*3C) and its frequencies in a sample of Iraqi
ALL paediatric patients. Methods: A cross sectional study was performed for 79 patients with Acute
lymphoblastic leukemia . Genotyping for (*3A , *3B , *3C ) the TPMT gene was performed by the
allele-specific multiplex-PCR analysis method. Results: The TPMT*3A mutant allele was found in 18
patients with allele frequency of (22.8 %), while TPMT*3C mutant allele was detected in 5 children
with allele frequency of 6.3% . But TPMT*3B mutant allele was not detected in whole sample ALL
patients .The correlation between gender and the polymorphism was not statically significant as p-value
0.23.. Conclusion: TMPT genotyping is an essential tool to reduce the cytotoxic effects of the anticancer
drug 6-MP in Iraqi paediatric patients with ALL .

Author Biographies

Esraa Ali Kadhum

Assist. Prof.,  College of Medicine, University of Baghdad, Baghdad, Iraq

Manal K Rasheed

Prof., College of Medicine, University of Baghdad, Baghdad, Iraq

Hasanein H.Ghali

Prof., College of Medicine, University of Baghdad, Baghdad, Iraq

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Published

2021-11-30

How to Cite

Esraa Ali Kadhum, Manal K Rasheed, & Hasanein H.Ghali. (2021). Thiopurine S-Methyltransferase Genotyping in Iraqi Childhood Acute Lymphoblastic Leukemia Patients ; A Single Institute Study. Indian Journal of Forensic Medicine & Toxicology, 16(1), 1647–1652. https://doi.org/10.37506/ijfmt.v16i1.17805