Changing of ATP and Its Metabolites in Blood Samples for Post Mortem Interval: In Vitro Study
Keywords:Post-mortem interval; ATP degradation; ATP metabolites; Forensic Science.
Background: Postmortem interval (PMI) is an important in forensic practice. Many studies in blood and tissue of
animals have revealed correlation between adenosine triphosphate (ATP) level and PMI. In this study aimed to
explore the correlation between human blood ATP and its metabolites level and PMI in vitro model.
Methods: Ten milliliter of venous blood samples were collected form four healthy men and contained in EDTA
tube. One milliliter of the blood sample was divided at 0, 1, 2, 4, 8, 12 and 24 h, respectively. The blood samples
were extracted and measured ATP, adenosine diphosphate (ADP) and adenosine monophosphate (AMP) level by
High performance liquid chromatography- diode array. Energy charge value was calculated to predict charge of
Results: The blood ATP, ADP and AMP levels increased in the first hour. The blood ATP, ADP and AMP levels
did not obviously change before 12 hour. The blood ATP level trended to decrease but the blood ADP and AMP
levels trended to increase after 24 hour. Energy Charge was not change in first 12 hour but trended to decrease
after 24 hour.
Conclusion: The blood ATP level and its metabolites changed after 12 hour and apply for the PMI investigation.
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