The Expression of Neogene TIGD3 that Derived from DNA Transposons in Colorectal Cancer Cell lines


  • Ahmed Arnaoty
  • Hiba. M. Ahmed
  • Yves Bigot
  • Thierry Lecomte



DNA transposons; domestication; neogene; TIGD3; microsatellite instable; microsatellite stable.


DNA transposons are exposed to a molecular domestication process, which results in the formation of neogenes,
which may play a role in human genetic instability. TIGD3 (Tigger-derived [TIGD] family of proteins) is one of
these Neogene, and its role in the human genome is unknown.
Aim: The expression of Neogene TIGD3 in colorectal cancer cell lines and its putative function in carcinogenesis
are being investigated.
Method: The protein expression of the TIGD3 gene was investigated using the western blot method in twelve
colorectal cancer cell lines (HCT116, SW48, LOVO, DLD1) that are microsatellite instable MSI, (SW480, SW620,
HT29, LS123, COLO205, T84, SW403, SW1463) that are microsatellite stable MSS, and in healthy colon tissue as a
control in our study.
Results: The expression of the TIGD3 protein was found in all twelve colorectal cancer cell lines, with varying
degrees of expression and numerous isoforms, which was not found in healthy colon tissue.
Conclusion: There may be a link between colorectal cancer evolution or progression and TIGD3 gene expression.

Author Biographies

Ahmed Arnaoty

Assistant Professor in Bilad AL-Rafidain University College Department of Anesthesia/Diyala/Iraq,

Hiba. M. Ahmed

2Lecturer in Al-Rasheed University College Department of Pharmacy/Baghdad/Iraq.

Yves Bigot

Director of research in UMR INRA-CNRS 7247, INRA Val de Loire-France,

Thierry Lecomte

Professor in Tours university, Department of Hepato- Gastro-Enterology; Tours/France.




How to Cite

Ahmed Arnaoty, Hiba. M. Ahmed, Yves Bigot, & Thierry Lecomte. (2022). The Expression of Neogene TIGD3 that Derived from DNA Transposons in Colorectal Cancer Cell lines. Indian Journal of Forensic Medicine & Toxicology, 16(3), 58–62.