Correlation of HIF-1α, CXCR4 and MMP13 Expression in Laryngeal Squamous Cell Carcinoma With Cervical Nodal Status

Authors

  • Etty Hary Kusumastuti
  • Anny Setijo Rahaju
  • Alphania Rahniayu
  • Sjahjenny Mustokoweni
  • Rovi Anggoro
  • Muhtarum Yusuf
  • I Ketut Sudiana

DOI:

https://doi.org/10.37506/ijfmt.v15i4.18426

Abstract

Background: Laryngeal carcinoma is the most common malignancy of the upper respiratory tract, more than 98% are squamous cell carcinoma (SCC). New method is necessary for identifying and αpredicting nodal metastasis in laryngeal SCC. Overproliferating tumor cells will induce hypoxia and release HIF-1α, which in turn will upregulate CXCR4. CXCR4 then will induce MMP13, a protein that degrade extracellular matrix (ECM), thus promoting metastatic process.

Methods: A cross sectional study, using 30 samples of laryngeal SCC, divided into two groups: based on cervical lymph node status. All samples were stained immunohistochemically against HIF-1α, CXCR4 and MMP13 antibody. The expressions were evaluated using immunoreactive score (IRS).

Result: There were significant difference among HIF1α, CXCR4, and MMP13 in laryngeal SCC with positive nodal metastasis group compared to negative nodal metastasis group (p<0,05).  

Conclusion: There were significant differences in HIF-1α, CXCR4 and MMP13 immunoexpression in laryngeal SCC with cervical nodal metastasis compared to laryngeal SCC without cervical nodal metastasis. There was a positive correlation between HIF-1α and MMP13 expression, positive correlation between CXCR4 and MMP13 expression, but there was no correlation between HIF-1α and CXCR4 expression.

Author Biographies

Etty Hary Kusumastuti

Researcher, Department of Anatomical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Dr. Soetomo General Academic Hospital, Surabaya, Indonesia

 

Anny Setijo Rahaju

Researcher, Department of Anatomical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Dr. Soetomo General Academic Hospital, Surabaya, Indonesia

Alphania Rahniayu

Researcher, Department of Anatomical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Dr. Soetomo General Academic Hospital, Surabaya, Indonesia

Sjahjenny Mustokoweni

Dr. Soetomo General Academic Hospital, Surabaya, Indonesia

Rovi Anggoro

Researcher, Department of Anatomical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Muhtarum Yusuf

Associate Professor, Department of Otholaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Dr. Soetomo General Academic Hospital, Surabaya, Indonesia

I Ketut Sudiana

Professor, Department of Anatomical Pathology, Faculty of Medicine Universitas Airlangga, Surabaya, Indonesia

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Published

2021-09-05

How to Cite

Etty Hary Kusumastuti, Anny Setijo Rahaju, Alphania Rahniayu, Sjahjenny Mustokoweni, Rovi Anggoro, Muhtarum Yusuf, & I Ketut Sudiana. (2021). Correlation of HIF-1α, CXCR4 and MMP13 Expression in Laryngeal Squamous Cell Carcinoma With Cervical Nodal Status. Indian Journal of Forensic Medicine & Toxicology, 15(4), 1086–1095. https://doi.org/10.37506/ijfmt.v15i4.18426