Correlation of HIF-1α, CXCR4 and MMP13 Expression in Laryngeal Squamous Cell Carcinoma With Cervical Nodal Status

Authors

  • Etty Hary Kusumastuti
  • Anny Setijo Rahaju
  • Alphania Rahniayu
  • Sjahjenny Mustokoweni
  • Rovi Anggoro
  • Muhtarum Yusuf
  • I Ketut Sudiana

DOI:

https://doi.org/10.37506/ijfmt.v15i4.18426

Abstract

Background: Laryngeal carcinoma is the most common malignancy of the upper respiratory tract, more than 98% are squamous cell carcinoma (SCC). New method is necessary for identifying and αpredicting nodal metastasis in laryngeal SCC. Overproliferating tumor cells will induce hypoxia and release HIF-1α, which in turn will upregulate CXCR4. CXCR4 then will induce MMP13, a protein that degrade extracellular matrix (ECM), thus promoting metastatic process.

Methods: A cross sectional study, using 30 samples of laryngeal SCC, divided into two groups: based on cervical lymph node status. All samples were stained immunohistochemically against HIF-1α, CXCR4 and MMP13 antibody. The expressions were evaluated using immunoreactive score (IRS).

Result: There were significant difference among HIF1α, CXCR4, and MMP13 in laryngeal SCC with positive nodal metastasis group compared to negative nodal metastasis group (p<0,05).  

Conclusion: There were significant differences in HIF-1α, CXCR4 and MMP13 immunoexpression in laryngeal SCC with cervical nodal metastasis compared to laryngeal SCC without cervical nodal metastasis. There was a positive correlation between HIF-1α and MMP13 expression, positive correlation between CXCR4 and MMP13 expression, but there was no correlation between HIF-1α and CXCR4 expression.

Author Biographies

  • Etty Hary Kusumastuti

    Researcher, Department of Anatomical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

    Dr. Soetomo General Academic Hospital, Surabaya, Indonesia

     

  • Anny Setijo Rahaju

    Researcher, Department of Anatomical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

    Dr. Soetomo General Academic Hospital, Surabaya, Indonesia

  • Alphania Rahniayu

    Researcher, Department of Anatomical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

    Dr. Soetomo General Academic Hospital, Surabaya, Indonesia

  • Sjahjenny Mustokoweni

    Dr. Soetomo General Academic Hospital, Surabaya, Indonesia

  • Rovi Anggoro

    Researcher, Department of Anatomical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

  • Muhtarum Yusuf

    Associate Professor, Department of Otholaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

    Dr. Soetomo General Academic Hospital, Surabaya, Indonesia

  • I Ketut Sudiana

    Professor, Department of Anatomical Pathology, Faculty of Medicine Universitas Airlangga, Surabaya, Indonesia

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Published

2021-09-05

How to Cite

Correlation of HIF-1α, CXCR4 and MMP13 Expression in Laryngeal Squamous Cell Carcinoma With Cervical Nodal Status. (2021). Indian Journal of Forensic Medicine & Toxicology, 15(4), 1086-1095. https://doi.org/10.37506/ijfmt.v15i4.18426