Trichloroethylene-induced Cellular Damage was Associated with Significant changes in the Concentrations of Caspase-3 and Glutathione in human lymphocytes

Authors

  • Malinee Pongsavee
  • Rajnikant Mishra

DOI:

https://doi.org/10.37506/ijfmt.v16i4.18607

Keywords:

Trichloroethylene, Lymphocyte, Cellular viability, Caspase-3 enzyme, Glutathione

Abstract

Trichloroethylene (TCE) is a volatile organic compound that is widely used in industries and potential source
of environmental contamination. The aims of this study were focused on the effects of TCE on cellular viability
and evaluation of critical markers like caspase-3 and glutathione (GSH) in human lymphocytes. The experiments
were studied in thirty volunteers. The TCE concentrations of 0.002, 0.004, 0.008, 0.016 and 0.032 mM/L were
treated in human lymphocytes for 48 h. Lymphocytes were cultured and effect of TCE on viability of lymphocytes
was studied by MTT assay. The expression of caspase-3 in TCE treated lymphocytes was studied by ELISA and
the concentration of glutathione in TCE treated lymphocytes was studied by colorimetric technique. The results
showed that, in the experimental groups at various TCE concentrations had lower cellular viability than the
control group (p<0.05). The caspase-3 concentration was increased when TCE concentration increased (p<0.05).
The glutathione concentration was decreased when TCE concentration increased (p<0.05). It is indicated that TCE
effected on cellular viability. The expression of caspase-3 enzyme and glutathione concentrations were changed
by TCE toxicity in lymphocytes.

Author Biographies

  • Malinee Pongsavee

    Associate Professor, Department of Medical Technology, Faculty of Allied Health Sciences,
    Thammasat University, Rangsit Campus, Patumthani, Thailand,

  • Rajnikant Mishra

    Professor,Biochemistry and Molecular Biology Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi, India

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Published

2022-10-10

How to Cite

Trichloroethylene-induced Cellular Damage was Associated with Significant changes in the Concentrations of Caspase-3 and Glutathione in human lymphocytes. (2022). Indian Journal of Forensic Medicine & Toxicology, 16(4), 313-319. https://doi.org/10.37506/ijfmt.v16i4.18607