Detection of Tetrahydrocannabinol in Commercial Consumables: A Survey-Based Study with Real Time Samples

Authors

  • Nupoor Gopal Neole Center for Nano and Material Science, JAIN (Deemed-to-be University)Jain Global Campus, Kanakapura, Ramanagaram, Bangalore, India
  • Anil Harishchandre

DOI:

https://doi.org/10.37506/7dd2kd70

Keywords:

Tetrahydrocannabinol, Cannabis, Cannabinoids, Dragendroff reagent, Fast blue B reagent, Oral consumable products.

Abstract

The study aimed to detect the presence of tetrahydrocannabinol (THC) in commercial consumables, 
specifically focusing on real-time samples. The study highlights the widespread use and cultivation 
of cannabis, with various compounds such as THC and Cannabidiol (CBD) present in the plant. THC is 
responsible for the psychoactive effects, while CBD is non-intoxicating and may have therapeutic 
benefits. The study also discusses the legal and social acceptance of cannabis across countries and 
regions. The study emphasizes the need for analysing street samples of pan-masala and other 
tobacco-containing orally consumable products for the presence of cannabis alkaloids, as they are 
often used as a concealment step for selling and purchasing cannabis. The experimental details 
include using chemicals and solvents from commercial sources, thin-layer chromatography (TLC) 
plates and an Ultraviolet-Visible spectrophotometer for detecting THC. The study used Dragendroff 
(DD) and Fast Blue B (FBB) reagents to identify THC. The THC quantities detected were 15.32 µM, 
29.2 µM, 7.15 µM, and 5.3 µM respectively in the collected sample.

Author Biographies

  • Nupoor Gopal Neole, Center for Nano and Material Science, JAIN (Deemed-to-be University)Jain Global Campus, Kanakapura, Ramanagaram, Bangalore, India

    Research Scholar, Center for Nano and Material Science, JAIN (Deemed-to-be University)Jain
    Global Campus, Kanakapura, Ramanagaram, Bangalore, India

  • Anil Harishchandre

    Assistant Professor, Institute of Forensic Science, RashtrasantTukadoji Maharaj Nagpur University, Nagpur, India.

References

(1) Mercolini, L.; Musenga, A.; Comin, I.; Baccini, C.; Conti, M.; Raggi, M. A. Determination of Plasma and Urine Levels of Δ9-Tetrahydrocannabinol and Its Main Metabolite by Liquid Chromatography after Solid-Phase Extraction. J. Pharm. Biomed. Anal.2008, 47 (1), 156–163. https://doi.org/10.1016/j.jpba.2007.12.023.

(2) Gambaro, V.; Dell’Acqua, L.; Farè, F.; Froldi, R.; Saligari, E.; Tassoni, G. Determination of Primary Active Constituents in Cannabis Preparations by High-Resolution Gas Chromatography/Flame Ionization Detection and High-Performance Liquid Chromatography/UV Detection. Anal. Chim. Acta2002, 468 (2), 245–254. https://doi.org/10.1016/S0003-2670(02)00660-8.

(3) Gul, W.; Gul, S. W.; Chandra, S.; Lata, H.; Ibrahim, E. A.; Elsohly, M. A. Detection and Quantification of Cannabinoids in Extracts of Cannabis Sativa Roots Using LC-MS/MS. Planta Med.2018, 84 (4), 267–271. https://doi.org/10.1055/s-0044-100798.

(4) Hidvégi, E.; Somogyi, G. P. Detection of Cannabigerol and Its Presumptive Metabolite in Human Urine after Cannabis Consumption. Pharmazie2010, 65 (6), 408–411. https://doi.org/10.1691/ph.2010.0035R.

(5) Dulaurent, S.; Gaulier, J. M.; Imbert, L.; Morla, A.; Lachâtre, G. Simultaneous Determination of Δ9-Tetrahydrocannabinol, Cannabidiol, Cannabinol and 11-nor-Δ9-Tetrahydrocannabinol-9-Carboxylic Acid in Hair Using Liquid Chromatography-Tandem Mass Spectrometry. Forensic Sci. Int.2014, 236, 151–156. https://doi.org/10.1016/j.forsciint.2014.01.004.

(6) Kintz, P.; Cirimele, V.; Ludes, B. Detection of Cannabis in Oral Fluid (Saliva) and Forehead Wipes (Sweat) from Impaired Drivers. J. Anal. Toxicol.2000, 24 (7), 557–561. https://doi.org/10.1093/jat/24.7.557.

(7) Madras, B. K. Update of Cannabis and Its Medical Use. Alcohol drug Abus. Res.2015, 5 (37), 1–41.

(8) Wolfgang Weinmann , Susanne Vogt, Rolf Goerke, Claudia Muller, A. B. Simultaneous Determination of THC-COOH and THC-COOH-Glucuronide in Urine Samples by LC/MS/MS. Forensic Sci. Int.2000, 113, 381–387. https://doi.org/https://doi.org/10.1016/S0379-0738(00)00210-3.

(9) Arena, P.; Rigano, F.; Guarnaccia, P.; Dugo, P.; Mondello, L.; Trovato, E. Elucidation of the Lipid Composition of Hemp (Cannabis Sativa L.) Products by Means of Gas Chromatography and Ultra-High Performance Liquid Chromatography Coupled to Mass Spectrometry Detection. Molecules2022, 27 (10). https://doi.org/10.3390/molecules27103358.

(10) Gill, A. D.; Hickey, B. L.; Zhong, W.; Hooley, R. J. Selective Sensing of THC and Related Metabolites in Biofluids by Host:Guest Arrays. Chem. Commun.2020, 56 (31), 4352–4355. https://doi.org/10.1039/d0cc01489c.

(11) Arkell, T. R.; Kevin, R. C.; Stuart, J.; Lintzeris, N.; Haber, P. S.; Ramaekers, J. G.; McGregor, I. S. Detection of Δ9 THC in Oral Fluid Following Vaporized Cannabis with Varied Cannabidiol (CBD) Content: An Evaluation of Two Point-of-Collection Testing Devices. Drug Test. Anal.2019, 11 (10), 1486–1497. https://doi.org/10.1002/dta.2687.

(12) Stevenson, H.; Bacon, A.; Joseph, K. M.; Gwandaru, W. R. W.; Bhide, A.; Sankhala, D.; Dhamu, V. N.; Prasad, S. A Rapid Response Electrochemical Biosensor for Detecting Thc In Saliva. Sci. Rep.2019, 9 (1), 1–11. https://doi.org/10.1038/s41598-019-49185-y.

(13) Wohlfarth, A.; Mahler, H.; Auwärter, V. Rapid Isolation Procedure for Δ9-Tetrahydrocannabinolic Acid A (THCA) from Cannabis Sativa Using Two Flash Chromatography Systems. J. Chromatogr. B Anal. Technol. Biomed. Life Sci.2011, 879 (28), 3059–3064. https://doi.org/10.1016/j.jchromb.2011.09.012.

(14) Wang, M.; Wang, Y. H.; Avula, B.; Radwan, M. M.; Wanas, A. S.; Mehmedic, Z.; van Antwerp, J.; ElSohly, M. A.; Khan, I. A. Quantitative Determination of Cannabinoids in Cannabis and Cannabis Products Using Ultra-High-Performance Supercritical Fluid Chromatography and Diode Array/Mass Spectrometric Detection. J. Forensic Sci.2017, 62 (3), 602–611. https://doi.org/10.1111/1556-4029.13341.

(15) Arkell, T. R.; Hayley, A. C.; Downey, L. A. Managing the High: Developing Legislation and Detection Methods for Cannabis Impairment. Nat. Rev. Neurosci.2021, 22 (9), 584. https://doi.org/10.1038/s41583-021-00500-5.

(16) Huestis, M. A.; Gustafson, R. A.; Moolchan, E. T.; Barnes, A.; Bourland, J. A.; Sweeney, S. A.; Hayes, E. F.; Carpenter, P. M.; Smith, M. L. Cannabinoid Concentrations in Hair from Documented Cannabis Users. Forensic Sci. Int.2007, 169 (2–3), 129–136. https://doi.org/10.1016/j.forsciint.2006.08.005.

(17) Laumon, B.; Gadegbeku, B.; Martin, J. L.; Biecheler, M. B. Cannabis Intoxication and Fatal Road Crashes in France: Population Based Case-Control Study. Br. Med. J.2005, 331 (7529), 1371–1374. https://doi.org/10.1136/bmj.38648.617986.1F.

(18) Hazekamp, A.; Peltenburg, A.; Verpoorte, R.; Giroud, C. Chromatographic and Spectroscopic Data of Cannabinoids from Cannabis Sativa L. J. Liq. Chromatogr. Relat. Technol.2005, 28 (15), 2361–2382. https://doi.org/10.1080/10826070500187558.

(19) Cary, P. The Marijuana Detection Window: Determining the Length of Time Cannabinoids Will Remain Detectable in Urine Following Smoking: A Critical Review of Relevant Research and Cannabinoid Detection Guidance for Drug Courts. Natl. Drug Court Inst.2006, 4 (2), 1–15.

(20) Michael J. Kogan, Eric Newman, N. J. W. Note Detection of Marijuana Metabolite 11-nor-A 9-Tetrahydrocannabinol-Q- Carboxylic Acid in Human Urine by Bonded-Phase Adsorption and Thin-Layer Chromatography. J. chromatoghraphy1984, 306, 441–443. https://doi.org/https://doi.org/10.1016/S0378-4347(00)80913-5.

(21) Beck, O.; Sandqvist, S.; Dubbelboer, I.; Franck, J. Detection of Δ 9-Tetrahydrocannabinol in Exhaled Breath Collected from Cannabis Users. J. Anal. Toxicol.2011, 35 (8), 541–544. https://doi.org/10.1093/anatox/35.8.541.

(22) Vollner, L.; Bieniek, D.; Korte, F. Review of Analytical Methods for Identification and Quantification of Cannabis Products. Regul. Toxicol. Pharmacol.1986, 6 (4), 348–358. https://doi.org/10.1016/0273-2300(86)90003-6.

(23) Smith, R. N. High Pressure Liquid Chromatography of Cannabis Identification of Separated Constituents. J. Chromatogr.1975, 115, 101–106. https://doi.org/https://doi.org/10.1016/S0021-9673(00)89021-4.

(24) Sherma, J.; Rabel, F. Thin Layer Chromatography in the Analysis of Cannabis and Its Components and Synthetic Cannabinoids. J. Liq. Chromatogr. Relat. Technol.2019, 42 (19–20), 613–628. https://doi.org/10.1080/10826076.2019.1663529.

(25) Lavanya, K.; Baggi, T. R. An Improved Thin-Layer Chromatographic Method for the Detection and Identification of Cannabinoids in Cannabis. Forensic Sci. Int.1990, 47 (2), 165–171. https://doi.org/10.1016/0379-0738(90)90210-P.

(26) Colette, A. NACD: The Potency of THC in Cannabis Products. Work. Pap. Ser.2011, 1 (1), 18.

(27) Pereira, J. F. Q.; Pimentel, M. F.; Amigo, J. M.; Honorato, R. S. Detection and Identification of Cannabis Sativa L. Using near Infrared Hyperspectral Imaging and Machine Learning Methods. A Feasibility Study. Spectrochim. Acta - Part A Mol. Biomol. Spectrosc.2020, 237, 118385. https://doi.org/10.1016/j.saa.2020.118385.

(28) dos Santos, N. A.; Souza, L. M.; Domingos, E.; França, H. S.; Lacerda, V.; Beatriz, A.; Vaz, B. G.; Rodrigues, R. R. T.; Carvalho, V. V.; Merlo, B. B.; Kuster, R. M.; Romão, W. Evaluating the Selectivity of Colorimetric Test (Fast Blue BB Salt) for the Cannabinoids Identification in Marijuana Street Samples by UV–Vis, TLC, ESI(+)FT-ICR MS and ESI(+)MS/MS. Forensic Chem.2016, 1, 13–21. https://doi.org/10.1016/j.forc.2016.07.001.

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Published

2024-04-27

How to Cite

Detection of Tetrahydrocannabinol in Commercial Consumables: A Survey-Based Study with Real Time Samples. (2024). Indian Journal of Forensic Medicine & Toxicology, 18(2), 92-97. https://doi.org/10.37506/7dd2kd70