Genotype and Haplotype of HLA- Class II in Type I and Type II Diabetes Mellitus in Iraqi Patients

Abstract

Background: Diabetes is one of the common complicated disease associated with multiple influenced factors, immunological serological and molecular factors play a great role in susceptibility and diagnosis of this disease. HLA genotyping were reported to have an influencing in both types of diabetes. Aim: The current study was conducted to investigate the association of human leukocytes genotypes in Diabetes patients. Method: Case-control study enrolled 225 blood samples collected from patient attended to the Marjan Teaching Hospital- Hilla and 25 apparently healthy from October 2018 to May 2019. Class II HLA genotyping was performing for 60 patients with diabetes mellitus and 25 healthy unrelated controls by means of the PCR-SSP method. The diagnosis of T1D and T1ID was set up according to American Diabetes Association criteria. Results: both types of diabetes were significantly associated with HLA-DR3. Associations were also observed with HLA –DQA105:01, the appearance of these two alleles differs in both TID and TIID . Conclusion. Certain HLA class II alleles, haplotypes, and genotypes have related to diabetes mellitus so it can be dependent as a genetic marker for susceptibility of this disease in Iraq.