Antifungal Activity of Crude and Phenolic Extract to Rice Crusts and Chemical Pesticide (Blitinute) in Inhibition of Fungi Isolate from Rice Seeds

Abstract

Objective: The major goal regarding the presented study is to evaluate the impact of the Rice extract from Rice Crusts and chemical pesticide (Blitinute) on the growth of pathogenic fungi. Method: Rice seed samples were collected directly from farmers field for isolating significant pathogenic fungi related to the seeds. Anti-fungal activity assay of the crude, Phenolic extract and chemical pesticide were determined by agar plating-method against some pathogenic fungi. Result: obtained results showed the effect concentration of rice seed extract on growth of fungi especially phenolic extract had wide spectrum antifungal activity than crude extract. when the use of chemical pesticide (Blitinute) at a concentration of 0.3% with reaching rate of inhibition to 100% to Fusarium graminerum , Penicillium viridicatum, while (88.8) the same concentration for the Aspergillus fumigates. In another than the use Integrations between two extract and chemical pesticide (Blitinute) to high effect against test fungi than one extract and chemical pesticide. Conclusion: This study demonstrates that the presence of three pathogenic fungi (Aspergillus fumigatus , Fusarium graminerium, Penicillium viridicatum) related to the rice grains have been controlled at various concentrations of two extract (crude & phenolic) and chemical pesticide totally inhibited radial growth regarding all test fungi .