Bacteriological Study of Klebsiella pneumoniae Isolated from Burn Patient in Al-Najaf City

Abstract

A total of (60) clinical specimens were collected from patients suffering from various burns during the period from) November 2018 to January, 2019). These specimens were collected from patients attending to Al-Furat Al-Awsat burn center during the studied period . All specimens were cultured on the MacConkey agar plates and incubated at 37°C under aerobic condition for 18 - 24 hour. In 60 patients, 36 (60%) were female and 24 (40%) were male. Several morphological, physiological and biochemical tests were made to identify bacterial isolates. Results showed that K.pneumoniae constitute 15 isolates (25%) of these isolates. A collection of 15 K. pneumoniae isolates diagnosed by the morphological ,cultural and biochemical characters, the identification was confirmed by molecular method for the presence of magA . The results showed that only 10 (66.6%) isolates was carrying magA which are diagnosed as K.pneumoniae. The results of antibiotic sensitivity screening test revealed that the isolates of K. pneumoniae exhibited highly sensitivity to Azithromycin ,moderate sensitivity for Gentamicin and Imipenem while the findings showed absolute resistant to Erythromycin , highly resistant to Ciprofloxacine , Trimthoprime. According to this result it can considered that Azithromycin as best treatment against K. pneumoniae.