Physiological and Gen Study for Animals Exposed to Leaves of Plant (Eucalyptus) from Selected Locations at, Al-Nassiriy/ Iraq

Abstract

Our study was carried out during the period from November 2018 to May 2019. 24 rats were used assigned and divided into three groups. The first group was the control group and a second group were injected with aqueous extraction of Eucalyptus leaves extract at concentrations of 100 and 150 mg /Kg experiment lasted 30 days. Zn, Pb, Cd, and Cu were measured in the leaves of plant and after confirming the presence of these heavy metals, we exposed the laboratory animals to the Eucalyptus aqueous extract and after the end of the period rats were sacrifice and blood collected. Blood were used to measure study parameters and relative gene expression of (SOD and catalase). Moreover, antioxidants and enzymes AST and AL were measured from serum. Results showed that the concentration of Pb in the plant was 0.19?g/g. The highest mean Zn was 14.23?g/g. The Cd concentration was 0.35?g/g and the copper concentration were 2.31?g/g from previous data we can find out that the amount of heavy metals is not really high. we would be expected low impact on animals since not high concentration found. The results of blood parameters of rats showed different outcome where it has been noticed that a significant increase in Hb, PLT and RBC at the 100mg/kg exposure and an increase in WBC in both concentrations. In addition, our results showed a significant decrease in RBC and Hb at the 150mg/Kg decrease MDA, MCV and MCH levels are decreased at both concentrations. A significant decrease in AST at 150mg/kg while the results showed a significant increase in lower concentration.