Elevated Corticosterone Level Due To Chronic Stress on  Hb-Egf Expression as a Marker of Endometrial Receptivity  Disorder in Rattus norvegicus

Authors

chorticosterone, chronic stress, HB-EGF expression, endometrial receptivity

Endometrial receptivity is a physiological state in which the endometrium obtains an adhesive phenotype

that allows embryo implantation and have a role in the problem of implantation failure. Endometrial

receptivity disorders can be caused by interference with the Hypothalamus-Pituitary-Gonad (HPG) axis

due to the activation of the Hypothalamus-Pituitary-Adrenal (HPA) axis by stress. HB-EGF is a biomarker

of endometrial receptivity that plays a role in the decidualization of endometrial stromal cells to reach the

receptive state and initiation of implantation. Corticosterone is the dominant glucocorticoid hormone in

rodents, as is cortisol in humans. High corticosterone due to chronic stress triggers disruption of homeostasis

in the endometrium by resulting in decreased levels of HB-EGF. This study aims to find out the effect

of increased corticosterone levels due to chronic stress on HB-EGF expression in endometrium Rattus

norvegicus. This research has obtained ethical eligibility from the Research Ethics Commission of the Faculty

of Medicine Airlangga University. The samples on this study were 34 rat (Rattus norvegicus) which were

divided into 2 groups, the control group and the stress treatment group using the Chronic Unpredictable Mild

Stress (CUMS) method. Corticosterone level were obtain from blood serum detected via ELISA and HB

EGF expression was obtained from endometrial in diestrus phase was evaluated by immunohistochemical

methods. Corticosterone levels in the stress treatment group were higher (72.84 ± 64.03) than in the control

group (23.29 ± 8.42). HB-EGF expression in the stress treatment group was lower (82.06 ± 5.91) than in

the control group (118.76 ± 13.20). Statistical tests showed significant differences in HB-EGF expression in

endometrium Rattus norvegicus p = 0,000 (p <0.05). Elevated level of corticosterone can decrease HB-EGF

expression in endometrium Rattus norvegicus.

Risya Secha Primindari1 , Ashon Sa’adi2 , Reny I’tishom3

1 Master degree of Reproductive Health Science, 2 Department of Obstetrics and Gynecology, 3Departemen of Biomedical Science, Faculty of Medicine Universitas Airlangga, Surabaya, Indonesia