Clinicomicrobiological Profile of Gram Negative Bacterial Infections in Adult ICU’s of a Tertiary Care Centre

Abstract

Objectives: To identify the common Gram negative bacteria associated with health care associated
infections,To study the antibiogram and to identify the presence of genes responsible for Carbapenem
resistance in Gram negative bacterial pathogens
Method: Prospective study was carried out for 2 years. The pathogens from cases of clinically suspected
infection in ICU were identified by the standard biochemical reactions. Antibiotic sensitivity testing was
done by the Modified Kirby-Bauer disk diffusion method . Multiplex PCR was done to detect the carbapenem
resistant genes in Gram negative bacilli :blaNDM-1, blaVIM, blaIMP,blaOXA-48 and blaKPC genes
Results: Study included 175 clinically significant Gram negative bacterial isolates .42% of the isolates
were from ET aspirate, followed by sputum,pus and blood. Klebsiella spp. and Acinetobacter spp. were the
common pathogens .18% of the pathogens showed Carbapenem resistance. The statistically significant risk
factors were Invasive procedure,ventilation,catheterization, prior antibiotic use, chronic liver,lung diseases
and diabetes. NDM 1 was the common gene identified.
Conclusion: Antibiotic resistance is on rise in our setup mandating continuous monitoring of the trend .