Development of Rapid, Sensitive and in-expensive Point of Care Diagnostic Method for Brucellosis in Dairy Cattle at Resource-Limited Areas
DOI:
https://doi.org/10.37506/ijphrd.v11i3.1229Keywords:
MRT (Milk Ring Test), RBPT (Rose Bengal Plate Agglutination test), Point of Care (POC), Polymerase Chain Reaction (PCR), Milk dipstick, iELISA (indirect Enzyme-Linked Immunosorbent Assay).Abstract
It is an emerging zoonotic disease spread all over the world, but its diagnosis is rather complex. Though a
number of serological tests are currently available for diagnosis, none of them are useful at POC diagnosis.
Further, they are not user-friendly and reproducible. Precisely for this reason, present study was aimed to
develop a simple, inexpensive, field applicable POC diagnostics using protein G-based milk dipstick and
indirect enzyme-linked immunosorbent (iELISA) assays. These are useful for rapid detection of brucellosis
using a handheld ELISA reader at resource-limited POC areas near pen side. Overall, 4998 raw milk and
whole blood samples were collected from organized dairy farms, where animals are reared (Goshalas),
and villages in different districts of Andhra Pradesh and Telangana states, India. Collected samples used to
evaluate diagnostic performances of these assays in comparison with milk ring test (MRT) and Rose Bengal
Plate Test (RBPT) serological assays. The developed milk dipstick and iELISA showed 87.37%, 99.67%
and 98.23%, 100% sensitivity and specificity respectively. Results proved that the developed assays can be
used as potential diagnostics for rapid field diagnosis of Brucellosis from infected animals using raw milk
and whole blood samples at resource-limited settings.
