Escherichia Coli as a Biological Model for Reduction of Graphene Oxide
DOI:
https://doi.org/10.37506/ijphrd.v11i3.1581Keywords:
Escherichia coli, Bacterial reduction of Graphene Oxide, NanobiotechnologyAbstract
The present study was conducted to investigate the ability of bacteria to reduce Graphene Oxide (GO)
sheets. Out of 82 bacterial isolates screened, one isolate was selected as a biological model for biosynthesis
of reduced graphene oxide (RGO) nanosheets. This isolate was identified by morphological, biochemical
tests, VITEK2 System, and 16S rRNA gene sequencing, aligned and submitted to the NCBI, and confirmed
as Escherichia coli strain E-NO.7 (accession no. MK685205). The UV-vis. absorption peak was around
284 nm. The SEM images revealed thin, wrinkled, closely associated nanosheets. The weight percentage of
carbon was 82.00% and oxygen was 18.00%. XRD analysis exhibited a broad diffraction peak at 2?= 26.5°
corresponding to the interlayer spacing of 0.34 nm. The FTIR spectrum showed decrease in peaks associated
to oxygen functional groups, while other peaks vanished completely.