Chemotactic Cytokine Receptor 5 Genetic Polymorphism in Diabetic Nephropathy of the Type 2 Diabetes Mellitus

Abstract

Background and Aim: The inflammatory process can be involved in the pathophysiology of diabetic
nephropathy (DN). One of the cytokine receptors in the inflammatory process is Chemotactic Cytokine
Receptor 5 (CCR5), whose genetic variation can affect the incidence of DN in several populations in
the world. This study aims to determine the proportion of CCR5 gene polymorphisms with DN in type
2 diabetes mellitus patients in Bali.
Method: This study used a case-control design in 144 DNA samples of patients with type 2 diabetes
mellitus. They were examined for the polymorphisms of the CCR5 gene by PCR-RFLP and then
analyzed using Chi-Square statistical test. The p value <0.05 was significant.
Results: The proportion of males more than females in subjects with diabetic nephropathy (p = 0.000).
Duration of diabetes and systolic blood pressure were higher in subjects with diabetic nephropathy (p
= 0.002; p = 0.002) respectively. The polymorphisms of the CCR5 gene did not differ significantly in
subjects with and without diabetic nephropathy (p = 0.224).
Conclusions: Gender, systolic blood pressure and duration of diabetes were associated with diabetic
nephropathy, while CCR5 gene polymorphisms were not significantly associated with diabetic
nephropathy in type 2 diabetes mellitus patients in Bali.