Correlation of Automated cell counters RBC Histogram and Peripheral smear in Anemias

Abstract

Background and Aim: The RBC histogram visualizes particle size distribution that plays a critical role in the initial
screening and detection method for haematological disorders in current clinical settings. This present study was designed
to determine the relationship between Abbott cell dyn ruby- 5 part analyzer automated haematology analyzer histograms
and peripheral smear using the blood samples.
Material and Methods: A total of 500 samples sent for CBC and PS would be used for the present study for the duration of
5 months. The CBC samples received would be analyzed in the ABOTT cell dyn ruby instrument, and a peripheral smear
would be made from the same sample, using Leishman stain.
Results: Among all, 16% histograms were normal,31% had a left-shifted curve,40% showed broad-based curve, 03%
showed short peak, and Bimodal peaked histogram was demonstrated by 06% of total cases. In the present study, cases of
dimorphic anemia showed a normal range of MCV, MCH and MCHC. At the same time, RDW is increased due to the high
degree of anisocytosis and poikilocytosis, which was observed in the PBS. Cases of Macrocytic anemia show an increase
in MCV, MCH and RDW with normal MCHC.
Conclusion: Histogram plays an additional role with peripheral smear for diagnosing RBCs disorders. Haematology
analyzers were very useful and reliable for evaluation of abnormal peripheral smears. Histogram was correlated with
almost all peripherial smear interpretation in anemia cases.