Evaluation of Vitek2 and Colistin broth disk elution test in comparison with Micro broth dilution for Susceptibility testing of Colistin among Carbapenem Resistant Enterobacterales.

Authors

  • Sujatha S R
  • Deepashree Rajasekhar
  • Satyasai. Badveti
  • Krishna Karthik Manthravadi

DOI:

https://doi.org/10.37506/ijphrd.v14i4.18632

Keywords:

Carbapenem resistant Enterobacterales, Colistin broth disk elution, rBMD- reference broth microdilution, Categorical Agreement.

Abstract

Colistin is the last expedient for treating the infections caused by Carbapenem resistant Enterobacter ales (CRE).
Expanding usage of colistin as led to development of Colistin resistance. This urges for a need of reliable and accurate
testing method that can be adopted as routine for susceptibility testing of Colistin. The innate cationic property of
colistin molecules is associated with complexities. Hence evaluation of testing method is needed for detection of colistin
resistance. Present study is done to evaluate the results yielding from Vitek2 and CBDE in comparison with rBMD. About
200 CRE isolated from clinical specimens were tested, results of Vitek2, CBDE was compared with rBMD. In comparison
with BMD Categorical agreement (CA) of Vitec 2 was 83% with major error of 17% .CA of CBDE was 99% with 1% of very
major error. Since the resistance to colistin is increasing accurate reporting of Colistin MIC by a validated method becomes
important. So, we would recommend CBDE for routine testing of Colistin susceptibility.

Author Biographies

Sujatha S R

Senior resident, Department of Microbiology, JSS Medical college, JSSAHER, Mysore, Karnataka

Deepashree Rajasekhar

Assistant Professor, Department of Microbiology, JSS Medical college, JSSAHER, Mysore, Karnataka

Satyasai. Badveti

Tutor & Research Scholar, Department of Microbiology, JSS Medical college, JSSAHER, Mysore, Karnataka

Krishna Karthik Manthravadi

Tutor & Research Scholar, Department of Microbiology, JSS Medical college, JSSAHER, Mysore, Karnataka

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Published

2022-10-10

How to Cite

Sujatha S R, Deepashree Rajasekhar, Satyasai. Badveti, & Krishna Karthik Manthravadi. (2022). Evaluation of Vitek2 and Colistin broth disk elution test in comparison with Micro broth dilution for Susceptibility testing of Colistin among Carbapenem Resistant Enterobacterales. Indian Journal of Public Health Research & Development, 13(4), 290–295. https://doi.org/10.37506/ijphrd.v14i4.18632