Bacterial Profile and Antimicrobial Susceptibility Pattern of Gram Negative Bacteria Isolated from Skin and Soft Tissue Infections in a Tertiary Care Hospital of Western Uttar Pradesh

Abstract

Background: Pyogenic infections are one of the most common causes of morbidity and mortality among
hospitalized patients. Gram negative bacteria (GNB)are predominantly isolated in Hospital settings across India.
A study was planned to determine the profile and susceptibility pattern of Gram negative bacteria isolated from
pus samples in a tertiary care Hospital.
Material and Methods: This prospective hospital based study was conducted over a period of one year. A total
of 1623 pus samples received in Clinical Microbiology Laboratory were subjected to culture and identification of
aerobic bacterial pathogen as per standard bacteriological method and antimicrobial susceptibility was carried out
by Kirby-Bauer disk diffusion method as per CLSI guidelines 2020.
Result: The culture positivity rate was 467(28.77%).There was predominance of GNB (78.59%). Klebsiella species
was the predominant GNB isolated. Most of the GNBs showed good susceptibility against imipenem. However,
lower susceptibility was observed against cephalosporins, quinolones, aminoglycosides and cotrimoxazole. None
of the isolated GNB exhibited resistance to colistin.
Conclusion: Klebsiella species was the predominant GNB isolated from pus samples from our hospital. Knowledge
of bacterial profile and their antimicrobial susceptibility pattern is important for institution of empirical
antimicrobial therapy for better patient outcome.