To Isolate and Identify Yeast Like Fungal Agents in Lower Respiratory Tract Sample among Patients Admitted in Intensive Care Unit
DOI:
https://doi.org/10.37506/5ts7hs12Keywords:
Fungal infection, Candida albicans, Non candida albicans, Candidiasis.Abstract
Context- To isolate and identify yeast like fungal agents in lower respiratory tract sample among patients admitted in intensive care unit.
Aim & Objectives- To isolate and identify yeast like fungal agents from the different lower respiratory tract samples up to the species level. And perform the antifungal sensitivity testing of
isolated species.
Material & Method-This study was conducted in the Mycology Section, Microbiology department, Moradabad (TMMC &RC), from November 2022 to June 2023. By using common microbiological techniques, Candida isolates were found in samples from the lower respiratory tract, and speciation was carried out using conventional and HiCrome differentiating medium. The disc diffusion technique developed by the CLSI(clinical and laboratory Standard Institute)was used to assess the antifungal susceptibility.
Result- 150 isolates were acknowledged from various clinical samples like BAL (Bronchoalveolar
Lavage), ET (Endotracheal tube) secretion, TT (Transtracheal) secretion. Where 49 (32%) were positive. Maximum age was 41-60 years and minimum age was from 0-20, from whom the isolates were discovered, among which females were (32.6%) and males were (65.3%). Prime Candida isolates were Candida albicans (44%), Candida tropicalis (45%). Other was Aspergillus fumigatus (60%)
Among the azoles, the most sensitive drug was Amphotericin B (95.83%) and least was fluconazole
(79.16%).
Conclusion- In comparison to Candida albicans, there is a varied tendency in the rising incidence of NAC (Non albicans Candida). Because some Candida species are naturally resistant to particular antifungal medicines, early detection of Candida strains would not only reduce the usage of empiric antifungal treatments but also have a significant impact on clinician’s treatment options and benefit
patients.
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