Carriage of Methicillin Resistant Staphylococcus Aureus among Healthcare Workers in a Tertiary Care Hospital
DOI:
https://doi.org/10.37506/30h2gq75Keywords:
Drug-resistant Staphylococcus aureus; Health care workers; Screening; decolonizationAbstract
Background: Methicillin-resistant S. aureus causes numerous illnesses, from relatively minor skin and soft tissue infections to potentially fatal systemic infections, are known to be caused by the multidrug-resistant bacterium. Methicillin resistance Staphylococcus aureus (MRSA) mostly spread through contact between patients and healthcare workers. Screening healthcare professionals who have been exposed to MRSA could help stop the spread of the organism. Aim- Carriage of Methicillin Resistant Staphylococcus aureus among health care workers in a tertiary care hospital. Objective- To detect Staphylococci from hand and nasal swab samples of health care workers (HCWs), to differentiate MRSA from isolated organism & to determine percentage of MRSA from different categories of HCWs.
Methods: Two samples were collected from 198 Health care workers, including samples from anterior nares and web spaces of both hands. MRSA and Staphylococcus aureus strains were identified by standard operating procedure.
Results: About 58(29.2%) were carriers of S. aureus and 20 (34.4%) were carriers of MRSA. Among the MRSA carriers, Nurses were 10 (50%) followed by doctors 5 (25%), Lab technicians were 4 (20%) & housekeeping staff 1(5%).
Conclusions: Despite having infection control policies in place, the MRSA carriage rate was higher in the nasal than in the hand. This signifies the importance of periodic systematic screening of all HCWs and decolonization, which may help eliminate the burden of MRSA carrier status and the spread of infection in the healthcare setting.
References
Bankar N, Wankhade A, Bramhane RB, Hathiwala R, & Chandi DH. Bacteriological Profile of PUS / WOUND SWAB andantimicrobial Susceptibility of Staphylococcus aureus isolated from of PUS & WOUND SWAB of Indoor Patients of Tertiary Care Hospital in Durg, Chhattisgarh India. International Journal of Innovative Research in Medical Science. 2018;3(04):1976-80.
Rajaduraipandi K, Mani KR, Panneerselvam K, Mani M, Bhaskar M, Manikandan P. Prevalence and antimicrobial susceptibility pattern of methicillin resistant Staphylococcus aureus: a multicentre study. Indian J Med Microbiol. 2006;24(1):34-8. PMID: 16505553.(6)
Patel SS, Bhawna, Sengupta C, Venkatesan A. Incidence of Methicillin Resistant Staphylococcus aureus in pus samples and associated risk factors in a tertiary care hospital. International Journal of Advanced Research. 2015;3(5):1097-1102 (7)
Ghias W, Sharif M, Ansari F, Rabbani M, Yazdani M. Isolation and identification of Methicillin and Vancomycin resistance Staphylococcus aureus from pus samples of injured skin patients in Lahore, Pakistan. Biomed Lett. 2016; 2(2):103-12.(8)
Ali Alghamdi B, Al-Shamrani JM, Musamed Alshamrani H, Al-Otaibi BG, Almazmomi K, Yusnoraini Yusof N, et.al. Antimicrobial resistance in methicillin-resistant Staphylococcus aureus. Saudi J Biol Sci. 2023;30(4):103604. PMID:36936699 (9)
Rupali S, Akshay R K, Nitin A A, Sarika P K. Prevalence of Methicillin Resistant Staphylococcus aureus in tertiary care hospital. Int J Microbiol App Sci. 2014;3(10):582-86. (10)
Yasmem F, Sarwar M I, Hakeem A, Sherwani K S, Hussein M S, Zeb M, et al. Identification of Staph aureus in pus samples and its antibiogram susceptibility again Imipenem, Tobramycin and Linezolid. Int J Bas Med Sci Pharm.2014;1(4):2049-4963.(11)
Clinical and Laboratory Standards Institute. Performance standards for Antimicrobial Susceptibility Testing. Twenty second Informational Supplement, CLSI document M100-S22 CLSI Wayne PA USA 2012 (12)
Chakrabarty S P, Mahapatra SK, Bal M, Roy S. Isolation and Identification Vancomycin resistance Staphylococcus aureus for post operative pus samples. J Med Sci. 2011;2(4):152-68.(14)
Mulqueen J, Cafferty F, Cormican F, Keane JD, Rossney A. Nasal carriage of Methicillin Resistant Staphylococcus aureus in GPs in the West of Ireland. Brit J General Practice. 2007;57(543):811-3. (PMID- 17925139) (17)
Akoua KC, Dje K, Toure R, Guessennd N, Acho B, Faye KH. Nasal Carriage of Methicillin-Resistant Staphylococcus aureus among health care personnel. Dakar Med. 2004; 49:70-4. (PMID-15782482) (18)
Albrich WC, Harbarth HS. Health-care workers: Source, Vector or victim of MRSA. Lancet Infect Dis. 2008; 8:289-301. (PMID- 18471774) (19)
Krishnakumar S, Krishnasamy L, Krishnagopal V, Saikumar C. Nasal carriage of Methicillin-Resistant Staphylococcus aureus among health care workers in a tertiary care hospital. Trop J Pathology Microbiol. 2019;5(11):878-83. (m&M)
Winn W, Allen S, Janda W, Koneman E, Procop G, Schreckenberger P, et.al. Koneman’s Color Atlas and Textbook of Diagnostic Microbiology, China:Sixth Edition. 1997:1443
Grace F. E-test for detection of Antimicrobial Susceptibility of Vancomycin in Methicillin Resistant Staphylococcus aureus (MRSA). J Pharm Bio Sci. 2013;3(7):29-31. (65)
Yazgi H, Ertek M. Nasal carriage of Staphylococcus aureus in hospital personnel and the normal population and antibiotic resistance of the isolates. Mikrobiyol Bul. 2003;37(2-3):137-42. (66)
Mukhiya RK, Shrestha A, Rai SK. Prevalence of methicillin-resistant Staphylococcus aureus in hospitals of Kathmandu Valley, Nepal. J Sci Technol. 2012; 13:185–90. (67)
Pant J, Rai SK. Occurrence of Staphylococcus aureus in hospital environment and staffs in teaching hospital in Kathmandu, Nepal. J Nepal Assoc Medi Lab Sci. 2007;8:72–3. (68)
Lepelletier D, Lucet JC. Controlling methicillin-susceptible Staphylococcus aureus: not simply methicillin-resistant S. aureus revisited. J Hosp Infect. 2013;84:13–21.(69)
Agarwal L, Singh AK, Sengupta C, Agarwal A. Nasal carriage of Methicillin- and Mupirocin-resistant S. aureus among health care workers in a tertiary care hospital. Journal of Research in Pharmacy Practice. 2015;4(4):182-86.(70)
Nadia.e.Al-Abdli,and Salch.H.Boiu Nasal carriage of Staphylococcus aures in health care workers in Bhenghazi Hospitals, Libya. American journal of microbiological research. 2014;2(4):110- 12.(71)
Shibabaw A, Abebe T, Mihret A. Antimicrobial susceptibility pattern of nasal Staphylococcus aureus among Dessie Referral Hospital health care workers, Dessie, Northeast Ethiopia. International Journal of Infectious Diseases. 2013;25(2):22-5.(72)
Khanal R, Sah P, Lamichhane P, Lamsal A, Upadhaya S, Pahwa VK. Nasal carriage of methicillin resistant Staphylococcus aureus among health care workers at a tertiary care hospital in Western Nepal. Antimicrobial Resistance and Infection Control. 2015;39(4):1-5.(73)
Rongpharpi SR, Hazarika NK, Kalita H. The Prevalence of Nasal Carriage of Staphylococcus Aureus Among Healthcare Workers at a Tertiary Care Hospital in Assam with Special Reference to MRSA. Journal of Clinical and Diagnostic Research 2013;7(2):257- 260 (74)
Shakya B, Shrestha S, Mitra T: Nasal carriage rate of methicillin resistant Staphylococcus aureus among at National Medical College Teaching Hospital, Birgunj. Nepal Med Coll J. 2010;12(1):26-29.(75)
Srinivasan A, Dick JD, Perl TM. Vancomycin Resistance in Staphylococci. Clin Microbiol Revie. 2002;3(15):430-38. (76)
Kumar A. Prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) In a Secondary Care Hospital in Northern Eastern Part of India. Arch Inf Dis Therapy. 2018;1(2):1-2.(50)
Harshan K H, Chavan K D. Prevalence and Susceptibility Pattern of Methicillin Resistant Staphylococcus aureus (MRSA) in Pus Samples at a Tertiary Care Hospital in Trivandrum. J Med Sci. 2015;4(11):718- 23.
Rao R, Basu R, Biswas DR. Aerobic bacterial profile and antimicrobial susceptibility pattern of pus isolates in a South Indian Tertiary Care Hospital. IOSR Journal of Dental and Medical Sciences. 2014;13(3):59-62.
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