Microbial Profile and Antibiotic susceptibility pattern of infectious agents isolated from patients admitted to intensive care unit of a tertiary care center at Bihta.

Authors

DOI:

https://doi.org/10.37506/bz54xf85

Keywords:

ICU, GNB, GPC, Antibiotics, Antibiotic resistance.

Abstract

Objectives :Patients with critical illness are admitted in intensive care units (ICU). A right antibiotics given to these patients timely, will help in their recovery. This study was done with an aim to know the profile of microorganism in the ICU setting and their antibiotic susceptibility pattern. Materials and methods :The study was conducted for 1 year period in a tertiary care hospital at Bihta, Patna. All the clinical samples received from various ICU’s were subjected to culture and sensitivity by standard microbiological procedures.Results :A total of 540 samples were received from various ICU’s. The culture positive rate in ICU was 22.8%. The most common culture positive sample received was blood 59 (48%). Among culture positive samples, 68 (55.3%) were Gram negative bacteria (GNB), followed by 43 (35%) Gram positive cocci (GPC) and 12 (9.7%) fungal isolates. Escherichia coli 28 (22.8%) was the most common isolates among GNB. Among GPC, the most common isolates was Coagulase negative Staphylococcus species (CONS) 32 (26%). All fungal isolates were Non albicans Candida (9.8%).Conclusion : This will help in making antibiotic policy of the ICU setting and will guide  cliniciansfor empirical therapy. It will prevent misuse of antibiotics and helps to combat antibiotic resistance.

Author Biographies

  • Anubhuti, Netaji Subhas Medical College and Hospital, Bihta, Patna, Bihar.

    Associate Professor, Department of Microbiology, Netaji Subhas Medical College and Hospital, Bihta, Patna, Bihar.

  • Mukesh kumar, Netaji Subhas Medical College and Hospital, Bihta, Patna, Bihar.

    Professor and Head, Department of Microbiology, Netaji Subhas Medical Collegeand Hospital, Bihta, Patna, Bihar.

  • Anupama Singh, Netaji Subhas Medical Collegeand Hospital, Bihta, Patna, Bihar.

    Associate Professor, Department of Microbiology, Netaji Subhas Medical Collegeand Hospital, Bihta, Patna, Bihar.

References

Sharma M, Sahai S, Sharma R. Bacteriological Profile and Antibiotic Susceptibility Pattern of Intensive Care Unit Patients in a Tertiary Care Institute: An Observational Study. Int Arch BioMed Clin Res. 2021;7(2):MB1–MB3

Jain AK, Jain SB, Ranjan KP, Misra V. Microbiological profile of infection in intensive care unit and their antimicrobial susceptibility pattern with special reference to metallo β-lactamases and AmpC. Int J Med Sci Public Health 2018;7(3):229-234

Banerjee B, Mondal S, Eshwara VK, Mukhopadhyay C, Varma M. Hospital Acquired Bloodstream Infection by Multi Drug Resistant Organisms-Alarming and Challenging Issue in the Intensive Care Units at Present Era. J Pure Appl Microbiol. 2020;14(4):2595-2601.

Chandi DH, Patil P, Bankar N, Damke S, Jain K. Antimicrobial Resistance Pattern of Bacterial Pathogens in Intensive Care Unit (ICU) of Tertiary Care Hospital. Indian Journal of Forensic Medicine &Toxicology, 2020;14(4):6396-6401.

C.J.L. Murray, K.S. Ikuta, F. Sharara, L.R. Swetschinski, G.R. Aguilar, A.P. Gray etal. Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis. Lancet, 399 (10325) (2022), pp. 629-655.

Sumanth Gandra, Jyoti Joshi, Anna Trett, Anjana SankhilLamkang, and Ramanan Laxminarayan. 2017. Scoping Report on Antimicrobial Resistance in India. Washington, DC: Center for Disease Dynamics, Economics & Policy.

Collee JG, Fraser AG, Marmion BP, Simmons A, editors. Mackie & McCartney’s Practical Medical Microbiology. 14th edition. New Delhi : Elsevier; 2012. 95-111.

Clinical and laboratory standards institute (2023). Performance standards for antimicrobial sensitivity testing. 33rd edition, CLSI supplement M100-s23, CLSI, Wayne.

SavanurSS , Gururaj H. Study of Antibiotic Sensitivity and Resistance Pattern of Bacterial Isolates in Intensive Care Unit Setup of a Tertiary Care Hospital. Indian Journal of Critical Care Medicine, 2019; 23(12):47-555.

N. Chidambaram, G. Ambujam, Reena Rajan, G. Sasikala, V. Anandi. Antimicrobial Profile of Clinical Isolates in Intensive Care Unit at a Tertiary Care Hospital. International Journal of Medical Research & Health Sciences, 2019, 8(2): 160-166.

Chandi DH, Patil P, Bankar N, Damke S, Jain K. Antimicrobial Resistance Pattern of Bacterial Pathogens in Intensive Care Unit (ICU) of Tertiary Care Hospital. Indian Journal of Forensic Medicine & Toxicology, October-December 2020, Vol. 14, No. 4 : 6396-6401.

Bhatnagar R, Patel P. Study of antimicrobial resistance pattern in blood isolates from critical care unit at a Tertiary Care hospital, Udaipur, Rajasthan, Indian Journal of Microbiology Research, January-March, 2018;5(1):61-65.

Saxena S, Priyadarshi M, Saxena A, Singh R. Antimicrobial consumption and bacterial resistance pattern in patients admitted in I.C.U at a tertiary care center. Journal of Infection and Public Health 12 (2019); 695–699.

David L. Horn, DionissiosNeofytos, Elias J. Anaissie, Jay A. Fishman, William J. Steinbach, Ali J. Olyaei, Kieren A. Marr, Michael A. Pfaller, Chi-Hsing Chang, Karen M. Webster. Epidemiology and Outcomes of Candidemia in 2019 Patients: Data from the Prospective Antifungal Therapy Alliance Registry. Clinical Infectious Diseases, Volume 48, Issue 12, 15 June 2009, 1695–1703.

Bhat V, Gupta S, et al. Bacteriological profile and antibiotic susceptibility patterns of clinical isolates in a tertiary care cancer centre. Indian J Med Paediatr Oncol 2016;37:204.

Nazneen S, Mukta K, et al. Bacteriological trends and antibiotic susceptibility patterns of clinical isolates at Government Cancer Hospital, Marathwada. Indian J Cancer 2016;53:583-586.

Gautam G, Satija S, Kaur R, Kumar A, Sharma Divakar, Dhakad MS. Insight into the Burden of Antimicrobial Resistance among Bacterial Pathogens Isolated from Patients Admitted in ICUs of a Tertiary Care Hospital in India. Canadian Journal of Infectious Diseases and Medical Microbiology. 2024;24:1-8

Moolchandani K, Sastry AS, Deepashree, Sistla S, Harish BN, Mandal J. Antimicrobial Resistance Surveillance among Intensive Care Units of a Tertiary Care Hospital in Southern India. Journal of Clinical and Diagnostic Research. 2017 Feb, Vol-11(2): DC01-DC07.

Sheth KV, Patel TK, Malek SS, Tripathi CB. Antibiotic sensitivity pattern of bacterial isolates from the intensive care unit of a tertiary care hospital in India. Tropical Journal of Pharmaceutical Research. 2012;11(6):991-99.

Kumar A, Raj N, Singh S, Das A, Singh V, Sen M, Agarwal J. A Retrospective Observational Study of the Microbial Etiology and Antimicrobial Susceptibility Patterns of Blood Cultures From ICU Patients at a Healthcare Facility in North India. 2024; Cureus 16(3): e57356.

Vanitha Rani N, K. Gopal, V. Narendra, V. Sunil, T. Palani. A retrospective study on blood stream infections and antibiotic susceptibility patterns in a tertiary care teaching hospital. International Journal of Pharmacy and Pharmaceutical Sciences. 2012; 4(1):543-548.

Chaturvedi P, Pandey A. Linezolid Resistant clinically Significant Isolates of Coagulase Negative Staphylococci: An Emerging Therapeutic Concern. Indian Journal of Public Health Research & Development, March 2020, Vol. 11, No. 03

Downloads

Published

2025-06-07

Issue

Vol. 16 No. 3 (2025): Indian Journal of Public Health and Research Development

Section

Original Article

License

Copyright (c) 2025 Anubhuti, Mukesh kumar, Anupama Singh

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

Microbial Profile and Antibiotic susceptibility pattern of infectious agents isolated from patients admitted to intensive care unit of a tertiary care center at Bihta. (2025). Indian Journal of Public Health Research & Development, 16(3), 21-27. https://doi.org/10.37506/bz54xf85