Molecular Screening of KI and WU Polyomaviruses among Patients with Chronic Kidney Disease and Urinary Tract Infections

Abstract

KIPyV and WUPyV were recognized based on commercial standard and PCR techniques. To occurrence
of KIPyV and WUPyV DNA among chronic kidney disease (CKD) and UTI patients using VP2 gene for
detection of KIPyV and VP1 gene for WUPyV. Molecular assay results of this study revealed the presence
of KIPyV in 6 (5.3%) of CKD patients and 3 (2.7%) of UTI patients while the presence of WUPyV was
1 (0.88%) in CKD patients and negative result in UTI patients. WUPyV DNA and KIPyV DNA was not
detected in plasma of healthy persons. The distribution of KIPyV and WUPyV according to gender among
CKD showed a no significance difference among both sexes in which (P value = 0.27) and (P value =
0.36) respectively while the distribution of KIPyV and WUPyV among urinary tract infections showed a no
significance difference among both sexes in which (P value = 0.13) and (P value = 0.79) respectively .The
current study survey about both viruses show that female was higher than male in KIPyV in which 2 (3.22%)
of KIPyV DNA were detected in male and 4 (7.84%) in female out of 113 (100%) among chronic kidney
disease patients while in urinary tract infection, patients revealed a higher percentage rate of KIPyV in
female than male in which 0 (0%) of KIPyV DNA were in male and 3 (4.54%) in female out of 111 (100%)
while doesn’t detect any isolate of WUPyV in both sexes.